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Mouse kappa Light chain antibody [RM103]

Mouse kappa Light chain antibody [RM103]. GTX60858
Mouse kappa Light chain antibody [RM103]. GTX60858
Mouse kappa Light chain antibody [RM103]. GTX60858

Cat No. GTX60858

Host Rabbit
Clonality Monoclonal
Clone Name RM103
Isotype IgG
Application WB, ICC/IF, FACS, IP, ELISA, IHC
Reactivity Mouse
Package
100 μg ($259)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG.
PROPERTIES

Form

Liquid

Buffer

PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse IgG

Target Isotype

IgG (kappa light chain)

Purification

Protein A affinity purified from an animal origin–free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Igk-C Antibody , IGKC Antibody , immunoglobulin kappa chain, constant region Antibody , MGC118128 Antibody
DATA IMAGES
Mouse kappa Light chain antibody [RM103]. GTX60858

GTX60858 ELISA Image

A titer ELISA of mouse IgG1κ. The plate was coated with different amounts of mouse IgG1κ. A serial dilution of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Mouse kappa Light chain antibody [RM103]. GTX60858

GTX60858 WB Image

Western blot of nonreduced(-) and reduced(+) mouse IgG1κ and IgG1λ (20ng/lane), using 0.2ug/mL of clone RM103. This antibody reacted to nonreduced IgG1κ (~150 kDa), and slightly react to reduced κ light chain (~25 kDa)

Mouse kappa Light chain antibody [RM103]. GTX60858

GTX60858 ELISA Image

ELISA of mouse immunoglobulins shows RM103 reacted to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

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Package List Price ($)
$ 259