GeneTex
United States (US)

NAK antibody

Cat No. GTX28121

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

For WB: Use at a dilution of 0.5 to 1 μg/ml and an84 kDa band should be detected. Optimal concentrations/dilutions should be determined by the researcher.

Calculated MW

84 kDa. ( Note )

Positive Control

MOLT-4 whole cell lysate

Specificity/Sensitivity

No cross reactivity to IKK alpha, IKK beta, IKK gamma, or IKK epsilon
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.24 with 0.02% sodium azide as preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: amino acids 712 to 727 of human NAK which are identical to those of mouse homologue

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tank Binding Kinase 1,Ftdals4,Iiae8,Nak,T2K,Tbk1

Cellular Localization

Cytoplasmic

Background

Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. Phosphorylation of I-kB by IkB kinase (IKK) complex leads to degradation of I-kB and activation of NF-kB. The IKK complex contains IKKa, IKKb, and IKKg. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-kB-activating kinase), and T2K (1-3). NAK/TBK1activates IKKb through direct phosphorylation. NAK/TBK1is activated by growth factors and PMA and mediates IKK and NF-kB activation in response to growth factors (2). NAK/TBK1 functions upstream of NIK and the IKK complex (1,2). NAK/TBK1 is also critical in protecting embryonic liver from apoptosis (3).

Database

Research Area

Package List Price ($)
$ 389