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NDRG1 antibody

Anti-NDRG1 antibody used in Western Blot (WB). GTX100537
Anti-NDRG1 antibody used in Western Blot (WB). GTX100537
Anti-NDRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100537
Anti-NDRG1 antibody used in Western Blot (WB). GTX100537

Cat. No. GTX100537

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NDRG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , HepG2 , NIH-3T3

Predict Reactivity

Bovine, Dog, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDRG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240553

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N-myc downstream regulated 1 , CAP43 , CMT4D , DRG-1 , DRG1 , GC4 , HMSNL , NDR1 , NMSL , PROXY1 , RIT42 , RTP , TARG1 , TDD5

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. It is necessary for p53-mediated caspase activation and apoptosis. Mutation in this gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDRG1 antibody used in Western Blot (WB). GTX100537

GTX100537 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
10% SDS PAGE

GTX100537 diluted at 1:1000

Anti-NDRG1 antibody used in Western Blot (WB). GTX100537

GTX100537 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NDRG1 antibody (GTX100537) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NDRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100537

GTX100537 IHC-P Image

NDRG1 antibody detects NDRG1 protein at membrane on human liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded liver carcinoma.
NDRG1 antibody (GTX100537) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDRG1 antibody used in Western Blot (WB). GTX100537

GTX100537 WB Image

NDRG1 antibody detects NDRG1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
NDRG1 antibody (GTX100537) dilution: 1:1000

REFERENCE

Application Reference

Tsai JS et al. PLoS One 2016; 11 (1) : e0147011 Cadmium Activates Multiple Signaling Pathways That Coordinately Stimulate Akt Activity to Enhance c-Myc mRNA Stability.
Application : WB
Reactivity : Human
Chang HY et al. J Proteome Res 2015; 14 (2) : 1250-62 Quantitative proteomics reveals middle infrared radiation-interfered networks in breast cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169