GeneTex
United States (US)

NEK7 (Active) recombinant protein

Cat No. GTX65461

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

221 nmol phosphate incorporated into beta-casein per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).

Calculated MW

63.0 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Storage

Store at -80ºC. Product is stable for at least 6-12 months.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Expression System

Baculovirus (Sf9 insect cells)

Purification


Purity was assessed by SDS-PAGE (≥90%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nima Related Kinase 7,Nek7

Background

Nek7 is a member of the NIMA (never in mitosis, gene A) family of serine/threonine kinases. In contrast to the other documented NIMA-related kinases, Nek7 harbor its catalytic domain in the C-terminus of the protein. Immunofluorescence studies suggest that Nek7 is cytoplasmic and located on chromosome 1 (1). During early embryogenesis Nek7 is expressed in the site of decidual reaction while later in embryogenesis, it is almost exclusively restricted to the nervous system in the dorsal thalamus (2). The major protein kinase that is active on the p70 S6 kinase hydrophobic regulatory site (FXXFS/TF/Y) Thr412 was purified from rat liver and identified as Nek7 (3). Nek7 kinase activity is rapidly and efficiently increased by serum deprivation, and may be regulated in a cell cycle-dependent manner.
Package List Price ($)
$ 349