50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
Store at -80ºC. Product is stable for at least 6-12 months.
0.1mg/ml(Please refer to the vial label for the specific concentration.)
Baculovirus (Sf9 insect cells)
Purity was assessed by SDS-PAGE (≥90%) and by HPLC.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Nima Related Kinase 7,Nek7
Nek7 is a member of the NIMA (never in mitosis, gene A) family of serine/threonine kinases. In contrast to the other documented NIMA-related kinases, Nek7 harbor its catalytic domain in the C-terminus of the protein. Immunofluorescence studies suggest that Nek7 is cytoplasmic and located on chromosome 1 (1). During early embryogenesis Nek7 is expressed in the site of decidual reaction while later in embryogenesis, it is almost exclusively restricted to the nervous system in the dorsal thalamus (2). The major protein kinase that is active on the p70 S6 kinase hydrophobic regulatory site (FXXFS/TF/Y) Thr412 was purified from rat liver and identified as Nek7 (3). Nek7 kinase activity is rapidly and efficiently increased by serum deprivation, and may be regulated in a cell cycle-dependent manner.