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NF-L antibody

Anti-NF-L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85460
Anti-NF-L antibody used in IHC (Paraffin sections) (IHC-P). GTX85460

Cat. No. GTX85460

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all NF-L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC 1:1000-1:2000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

300 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the NF-L gene product, but are shared between the human (P07196, NCBI) and mouse (P08551, NCBI) protein sequences.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurofilament light , CMT1F , CMT2E , CMTDIG , NF-L , NF68 , NFL , PPP1R110

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-NF-L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85460

GTX85460 ICC/IF Image

ICC/IF analysis of mouse brain cortical neurons using GTX85460 NF-L antibody.
Red and Yellow : Primary antibody
Blue : DAPI
Dilution : 1:1000

Anti-NF-L antibody used in IHC (Paraffin sections) (IHC-P). GTX85460

GTX85460 IHC-P Image

IHC-P analysis of mouse cerebellum using GTX85460 NF-L antibody.
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399