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NFAT1 antibody

Anti-NFAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33354
Anti-NFAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33354
Anti-NFAT1 antibody used in Western Blot (WB). GTX33354

Cat. No. GTX33354

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NFAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:50 - 1:200
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human NFATC2

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor of activated T cells 2 , NFAT1 , NFATP

Cellular Localization

Cytoplasm,Nucleus

Background

This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-NFAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33354

GTX33354 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX33354 NFAT1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-NFAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33354

GTX33354 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX33354 NFAT1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-NFAT1 antibody used in Western Blot (WB). GTX33354

GTX33354 WB Image

WB analysis of various sample lysates using GTX33354 NFAT1 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:500
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399