GeneTex

  • Country / Location Selection

United States (US)

NFAT1 (phospho Ser54) antibody

Orthogonal
Anti-NFAT1 (phospho Ser54) antibody used in Western Blot (WB). GTX25246
Anti-NFAT1 (phospho Ser54) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25246
Anti-NFAT1 (phospho Ser54) antibody used in Western Blot (WB). GTX25246

Cat. No. GTX25246

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all NFAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:250
FACS 1:20
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of mouse NFAT1 that contains serine 54.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380487

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 , AI607462 , NF-ATc2 , NF-ATp , NFAT1 , NFAT1-D , Nfatp

Cellular Localization

Cytoplasm,Nucleus

Background

Nuclear factor of activated T cells (NFAT) is a family of transcription factors implicated in multiple biological processes including cytokine gene expression, cardiac hypertrophy and adipocyte differentiation. NFAT 1 (also known as NFATc2 or NFATp) is a 154 kDa member of this family that is regulated by the calcium-dependent phosphatase calcineurin. When calcineurin is activated by calcium it dephosphorylates multiple residues in the regulatory domain of NFAT 1, leading to its translocation to the nucleus and activation of its transcriptional activity. Once in the nucleus, NFAT proteins act synergistically with the AP-1 transcription factor complex to regulate the expression of multiple genes. Serine 54 in mouse NFAT 1 has been shown to be important in the regulation of its transcriptional activity.

Database

Research Area

DATA IMAGES

Anti-NFAT1 (phospho Ser54) antibody used in Western Blot (WB). GTX25246

GTX25246 WB Image

Orthogonal

WB analysis of murine T cells treated with PMA and Ca²⁺ ionophore ionomycin (Lane 2-5) using GTX25246 NFAT1 (phospho Ser54) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine containing peptide(Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

Anti-NFAT1 (phospho Ser54) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25246

GTX25246 ICC/IF Image

Orthogonal

ICC/IF analysis of HeLa cells treated with 200nM of PMA for 20 minutes using GTX25246 NFAT1 (phospho Ser54) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

Anti-NFAT1 (phospho Ser54) antibody used in Western Blot (WB). GTX25246

GTX25246 WB Image

Orthogonal

WB analysis of whole cell extracts (30 μg lysate) of NIH/3T3 (Lane 1) treated for 20 minutes with 200 nM of PMA (Lane 2), Jurkat (Lane 3) treated for 20 minutes with 200 nM of PMA (Lane 4) using GTX25246 NFAT1 (phospho Ser54) antibody.
Dilution : 1:500-1:2000

REFERENCE

Application Reference

Banerjee A et al. JCI Insight 2022; 7 (10) A reengineered common chain cytokine augments CD8+ T cell-dependent immunotherapy.
Application : WB
Reactivity : Mouse
So JS et al. J Immunol 2014; 193 (6) : 2772-83 6-methoxyflavone inhibits NFAT translocation into the nucleus and suppresses T cell activation.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for NFAT1 (phospho Ser54) antibody (GTX25246). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399