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NFAT2 antibody [7A6]

Orthogonal
Anti-NFAT2 antibody [7A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22796
Anti-NFAT2 antibody [7A6] used in IHC (Paraffin sections) (IHC-P). GTX22796
Anti-NFAT2 antibody [7A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22796
Anti-NFAT2 antibody [7A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22796
Anti-NFAT2 antibody [7A6] used in IHC (Paraffin sections) (IHC-P). GTX22796
Anti-NFAT2 antibody [7A6] used in IHC (Paraffin sections) (IHC-P). GTX22796

Cat. No. GTX22796

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7A6

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay, Gel supershift assays

Reactivity

 Human, Mouse, Rat, Rabbit, Hamster, Primate
Package
100 μl ($399)
See all NFAT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2,000
ICC/IF 1:10-1:250
IHC-P Assay dependent
IP Assay dependent
ChIP assay Assay dependent
Gel supershift assays Assay dependent
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Product Note

This antibody does not cross react with NFATc2 (NFAT1).

PROPERTIES

Form

Liquid

Buffer

Ascites diluted with PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor of activated T cells 1 , NF-ATC , NF-ATc1.2 , NFAT2 , NFATc

Cellular Localization

Cytoplasm,Nucleus

Background

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-NFAT2 antibody [7A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22796

GTX22796 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22796 NFAT2 antibody [7A6]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-NFAT2 antibody [7A6] used in IHC (Paraffin sections) (IHC-P). GTX22796

GTX22796 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX22796 NFAT2 antibody [7A6].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-NFAT2 antibody [7A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22796

GTX22796 ICC/IF Image

ICC/IF analysis of 293 cells using GTX22796 NFAT2 antibody [7A6]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-NFAT2 antibody [7A6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22796

GTX22796 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX22796 NFAT2 antibody [7A6]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-NFAT2 antibody [7A6] used in IHC (Paraffin sections) (IHC-P). GTX22796

GTX22796 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22796 NFAT2 antibody [7A6].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-NFAT2 antibody [7A6] used in IHC (Paraffin sections) (IHC-P). GTX22796

GTX22796 IHC-P Image

IHC-P analysis of human spleen tissue using GTX22796 NFAT2 antibody [7A6].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

REFERENCE

Application Reference

Zhu X et al. J Orthop Surg Res 2022; 17 (1) : 423 ZEB1 regulates bone metabolism in osteoporotic rats through inducing POLDIP2 transcription.
Application : WB
Reactivity : Mouse
Patil S et al. Cells 2021; 10 (12) Chromatin-Independent Interplay of NFATc1 and EZH2 in Pancreatic Cancer.
Application : ChIP assay
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399