APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 3 μg/ml |
| 1:20 |
Not tested in other applications.
Calculated MW
Product Note
The amino acid sequence used is 90% homologous in mouse and rat and 100% identical in human NFATc1 A, B, C and E isoforms. The reported amino acid size of NFATc1 isoforms are as follows: A -716 aa, B-825 aa, C-930 aa, and E-812 aa.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Amino acids 264-282 (NKRKYSLNGRQPPYSPHHS) of human NFATc1 were used as immunogen for this antibody.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear factor of activated T cells 1 , NF-ATC , NF-ATc1.2 , NFAT2 , NFATc
Cellular Localization
Cytoplasm,Nucleus
Background
The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX45916 WB Image
WB analysis of Ramos (A) and RAW264.7 (B) cell lysate using GTX45916 NFAT2 antibody.
Dilution : 1 μg/ml
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GTX45916 ICC/IF Image
ICC/IF analysis of RAW264.7 cells using GTX45916 NFAT2 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:10
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GTX45916 WB Image
WB analysis of Ramos cell lysate using GTX45916 NFAT2 antibody.
Lane 1 : Without blocking peptide
Lane 2 : With blocking peptide
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GTX45916 WB Image
WB analysis of RAW264.7 cell lysate using GTX45916 NFAT2 antibody.
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REFERENCE
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REVIEW
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