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NFIA antibody

Orthogonal
Anti-NFIA antibody used in IHC (Frozen sections) (IHC-Fr). GTX114580
Anti-NFIA antibody used in Western Blot (WB). GTX114580
Anti-NFIA antibody used in IHC (Paraffin sections) (IHC-P). GTX114580
Anti-NFIA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114580
Anti-NFIA antibody used in IHC (Paraffin sections) (IHC-P). GTX114580
Anti-NFIA antibody used in IHC (Paraffin sections) (IHC-P). GTX114580

Cat. No. GTX114580

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NFIA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Predict Reactivity

Zebrafish, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFIA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885343

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor I A , BRMUTD , CTF , NF-I/A , NF1-A , NFI-A , NFI-L

Cellular Localization

Nucleus

Background

Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NFIA antibody used in IHC (Frozen sections) (IHC-Fr). GTX114580

GTX114580 IHC-Fr Image

NFIA antibody detects NFIA protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: NFIA protein stained by NFIA antibody (GTX114580) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NFIA antibody used in Western Blot (WB). GTX114580

GTX114580 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NFIA antibody (GTX114580) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFIA antibody used in IHC (Paraffin sections) (IHC-P). GTX114580

GTX114580 IHC-P Image

NFIA antibody detects NFIA protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
NFIA antibody (GTX114580) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NFIA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114580

GTX114580 ICC/IF Image

NFIA antibody detects NFIA protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFIA protein stained by NFIA antibody (GTX114580) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-NFIA antibody used in IHC (Paraffin sections) (IHC-P). GTX114580

GTX114580 IHC-P Image

NFIA antibody detects NFIA protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
NFIA antibody (GTX114580) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NFIA antibody used in IHC (Paraffin sections) (IHC-P). GTX114580

GTX114580 IHC-P Image

NFIA antibody detects NFIA protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
NFIA antibody (GTX114580) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169