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NFIB antibody [N1C2]

Anti-NFIB antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX105749
Anti-NFIB antibody [N1C2] used in Western Blot (WB). GTX105749
Anti-NFIB antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX105749
Anti-NFIB antibody [N1C2] used in Western Blot (WB). GTX105749

Cat. No. GTX105749

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NFIB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Mouse brain , NFIB transfected 293T

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFIB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950994

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor I B , CTF , HMGIC/NFIB , MACID , NF-I/B , NF1-B , NFI-B , NFI-RED , NFIB2 , NFIB3

Cellular Localization

Nucleus

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Database

Research Area

DATA IMAGES

Anti-NFIB antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX105749

GTX105749 IHC-P Image

NFIB antibody [N1C2] detects NFIB protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
NFIB antibody [N1C2] (GTX105749) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NFIB antibody [N1C2] used in Western Blot (WB). GTX105749

GTX105749 WB Image

Western Blot analysis of NFIB expression in transfected 293T cell line by NFIB polyclonal antibody.
A: Non-transfected lysate.
B: NFIB transfected lysate.
12% SDS PAGE
GTX105749 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFIB antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX105749

GTX105749 IHC-P Image

NFIB antibody [N1C2] detects NFIB protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
NFIB antibody [N1C2] (GTX105749) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NFIB antibody [N1C2] used in Western Blot (WB). GTX105749

GTX105749 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NFIB antibody [N1C2] (GTX105749) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169