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NFIX antibody [N2C2], Internal

Anti-NFIX antibody [N2C2], Internal used in Western Blot (WB). GTX118630
Anti-NFIX antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX118630
Anti-NFIX antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118630
Anti-NFIX antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX118630

Cat. No. GTX118630

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NFIX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Predict Reactivity

Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFIX. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618175

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor I X , CTF , MRSHSS , NF-I/X , NF1-X , NF1A , SOTOS2

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Database

Research Area

DATA IMAGES

Anti-NFIX antibody [N2C2], Internal used in Western Blot (WB). GTX118630

GTX118630 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NFIX antibody [N2C2], Internal (GTX118630) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NFIX antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX118630

GTX118630 IHC-P Image

NFIX antibody [N2C2], Internal detects NFIX protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat hippocampus.
NFIX stained by NFIX antibody [N2C2], Internal (GTX118630) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NFIX antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118630

GTX118630 ICC/IF Image

NFIX antibody [N2C2], Internal detects NFIX protein by immunofluorescent analysis.
Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFIX stained by NFIX antibody [N2C2], Internal (GTX118630) diluted at 1:500.
Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NFIX antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX118630

GTX118630 IHC-P Image

NFIX antibody [N2C2], Internal detects NFIX protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
NFIX stained by NFIX antibody [N2C2], Internal (GTX118630) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169