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NFkB p105 antibody

KO/KD
Anti-NFkB p105 antibody used in IHC (Paraffin sections) (IHC-P). GTX35196
Anti-NFkB p105 antibody used in Western Blot (WB). GTX35196
Anti-NFkB p105 antibody used in Western Blot (WB). GTX35196
Anti-NFkB p105 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35196

Cat. No. GTX35196

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all NFkB p105 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 740-964 of human NFKB1 (NP_001158884.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor kappa B subunit 1 , CVID12 , EBP-1 , KBF1 , NF-kB1 , NF-kappa-B1 , NF-kappaB , NFKB-p105 , NFKB-p50 , NFkappaB , p105 , p50

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-NFkB p105 antibody used in IHC (Paraffin sections) (IHC-P). GTX35196

GTX35196 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX35196 NFkB p105 antibody.
Dilution : 1:200

Anti-NFkB p105 antibody used in Western Blot (WB). GTX35196

GTX35196 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35196 NFkB p105 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-NFkB p105 antibody used in Western Blot (WB). GTX35196

GTX35196 WB Image

WB analysis of HeLa cell lysate using GTX35196 NFkB p105 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-NFkB p105 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35196

GTX35196 ICC/IF Image

ICC/IF analysis of A549 cells using GTX35196 NFkB p105 antibody.

REFERENCE

Application Reference

Song L et al. Brain Res Bull 2021; 177 : 81-91 Antidepressant effect of catalpol on corticosterone-induced depressive-like behavior involves the inhibition of HPA axis hyperactivity, central inflammation and oxidative damage probably via dual regulation of NF-κB and Nrf2.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399