GeneTex
United States (US)

NGF antibody [AS18] (Biotin)

Cat No. GTX16160

Host Mouse
Clonality Monoclonal
Clone Name AS18
Isotype IgG1
Application ELISA
Reactivity Human, Mouse, Bovine
APPLICATION

Application Note

ELISA: Use at a concentration of 2 μg/ml. Typically for human NGF, the ELISA has a working range of 0-15 ng/ml and a sensitivity of 15 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 μl. Recovery of human NGF in human plasma is typically > 90% in the ELISA. The use of Tris buffer, pH 7, instead of PBS is recommended. Optimal dilutions/concentrations should be determined by the end user.

Specificity/Sensitivity

GTX16160 recognises human 2.5S, 7S and bNGF.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.08% Sodium Azide; Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Unfortunately, this information is considered to be commercially sensitive

Purification

Protein G purified
This antibody is provided as a 0.2μm sterile filtered solution. It shows >95% purity by SDS PAGE.

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Secreted

Background

Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture NGF induces the formation of neurite projections and in vivo may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease.

Research Area

Package List Price ($)
$ 319