ELISA: Use at a concentration of 2 μg/ml. Typically for human NGF, the ELISA has a working range of 0-15 ng/ml and a sensitivity of 15 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 μl. Recovery of human NGF in human plasma is typically > 90% in the ELISA. The use of Tris buffer, pH 7, instead of PBS is recommended. Optimal dilutions/concentrations should be determined by the end user.
GTX16160 recognises human 2.5S, 7S and bNGF.
Preservative: 0.08% Sodium Azide; Constituents: PBS
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Unfortunately, this information is considered to be commercially sensitive
Protein G purified
This antibody is provided as a 0.2μm sterile filtered solution. It shows >95% purity by SDS PAGE.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture NGF induces the formation of neurite projections and in vivo may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease.