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NNT1 antibody

Anti-NNT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114173
Anti-NNT1 antibody used in Western Blot (WB). GTX114173
Anti-NNT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114173

Cat. No. GTX114173

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NNT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

human CLCF1-transfected 293T cells

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NNT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620783

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cardiotrophin like cytokine factor 1 , BSF-3 , BSF3 , CISS2 , CLC , NNT-1 , NNT1 , NR6

Cellular Localization

Secreted

Background

CLCF1 belongs to the interleukin-6 (IL6; MIM 147620) family of cytokines, which are involved in cell signaling through phosphorylation of gp130 (IL6ST; MIM 600694). IL6 family members share similarity in gene structure and have a 4-helix bundle in their protein structure.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NNT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114173

GTX114173 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using NNT1(GTX114173) antibody at 1:200 dilution.

Anti-NNT1 antibody used in Western Blot (WB). GTX114173

GTX114173 WB Image

NNT-1/BSF-3 antibody detects CLCF1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human CLCF1-transfected 293T cells
12 % SDS-PAGE
NNT-1/BSF-3 antibody (GTX114173) dilution: 1:5000

Anti-NNT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114173

GTX114173 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NNT-1/BSF-3(GTX114173) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169