NOL5A antibody

Cat. No. GTX130973

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all NOL5A products

Datasheet
Document

View product citations for antibody GTX130973 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus of human NOL5A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886399

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NOP56 ribonucleoprotein , NOL5A , SCA36

Cellular Localization

Nucleus , nucleolus

Background

Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by this gene is similar in sequence to Nop56p and is also found in the nucleolus. Expansion of a GGCCTG repeat from 3-8 copies to 1500-2500 copies in an intron of this gene results in spinocerebellar ataxia 36. Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of most of them has not been determined. [provided by RefSeq, Jul 2016]

Database

Gene ID: 10528 NOP56
UniProt: O00567 NOP56

Research Area

DATA IMAGES

GTX130973 IHC-P Image

NOP56 antibody detects NOP56 protein at cytoplasm and nucleus in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NOP56 antibody (GTX130973) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX130973 IP Image

Immunoprecipitation of NOP56 protein from 293T whole cell extracts using 5 μg of NOP56 antibody (GTX130973).
Western blot analysis was performed using NOP56 antibody (GTX130973) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX130973 ICC/IF Image

NOP56 antibody detects NOP56 protein at nucleolus and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NOP56 protein stained by NOP56 antibody (GTX130973) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX130973 WB Image

NOP56 antibody detects NOP56 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOP56 antibody (GTX130973) diluted at a dilution of 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty