GeneTex
United States (US)

NOTCH2 (cleaved Asp1733) antibody

Notch-2-cleaved-Asp733-antibody-GTX86955-WB-1_18121411_573.jpg

Cat No. GTX86955

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:
WB: 1:500~1:1000
IHC-P: 1:50~1:100
ELISA: 1:40000
Optimal dilutions should be determined experimentally by the end user.

Calculated MW

100 and 130 kDa. ( Note )

Specificity/Sensitivity

NOTCH2 (Cleaved-Asp1733) Antibody detects endogenous levels of fragment of activated Notch 2 resulting from cleavage adjacent to Asp1733.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human NOTCH2.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Notch 2,Ags2,Hjcys,Hn2,Notch2

Cellular Localization

Following proteolytical processing NICD is translocated to the nucleus.

Background

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Database

Research Area

DATA IMAGES
Notch-2-cleaved-Asp733-antibody-GTX86955-WB-1_18121411_573.jpg

GTX86955 WB Image

Western blot analysis of extracts from 293 cells, treated with TNF-a 20ng/ml 30', using Notch 2 (Cleaved-Asp1733) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339