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NOX2 / gp91phox antibody

Anti-NOX2 / gp91phox antibody used in Western Blot (WB). GTX12024
Anti-NOX2 / gp91phox antibody used in Western Blot (WB). GTX12024
Anti-NOX2 / gp91phox antibody used in IHC (Paraffin sections) (IHC-P). GTX12024

Cat. No. GTX12024

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($449)
See all NOX2 / gp91phox products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml
Not tested in other applications.

Calculated MW

65.34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1% Na₂HPO₄, 0.45% NaCl, 2.5% BSA

Preservative

0.025% Thimerosal, 0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NOX2(442-459aa YWLCRDTHAFEWFADLLQ), identical to the related rat and mouse sequences.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cell membrane

Background

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NOX2 / gp91phox antibody used in Western Blot (WB). GTX12024

GTX12024 WB Image

WB analysis of various samples using GTX12024 NOX2 / gp91phox antibody.
Lane 1 : rat thymus tissue lysates
Lane 2 : rat brain tissue lysates
Dilution : 0.5 μg/mL
Loading : 50μg of sample under reducing conditions

Anti-NOX2 / gp91phox antibody used in Western Blot (WB). GTX12024

GTX12024 WB Image

WB analysis of various samples using GTX12024 NOX2 / gp91phox antibody.
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : SMMC cell lysate

Anti-NOX2 / gp91phox antibody used in IHC (Paraffin sections) (IHC-P). GTX12024

GTX12024 IHC-P Image

IHC-P analysis of human intestinal cancer tissue using GTX12024 NOX2 / gp91phox antibody.

REFERENCE

Application Reference

Wei X et al. Medicine (Baltimore) 2022; 101 (32) : e30028 Increased NOX1 and DUOX2 expression in the colonic mucosa of patients with chronic functional constipation.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 449