APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 2 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Rat Brain Tissue Lysate(GTX27942)
Product Note
At least two isoforms are known to exist; this antibody will detect both isoforms. NOX2 is predicted to not cross-react with other NOX proteins.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2.The immunogen is located within amino acids 140 - 190 of NOX2.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytochrome b-245 beta chain , AMCBX2 , CGD , GP91-1 , GP91-PHOX , GP91PHOX , IMD34 , NOX2 , p91-PHOX
Cellular Localization
Cell membrane; Multi-pass membrane protein
Background
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31930 IHC-P Image
IHC-P analysis of rat brain tissue using GTX31930 NOX2 / gp91phox antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml
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GTX31930 WB Image
WB analysis of rat brain tissue lysate using GTX31930 NOX2 / gp91phox antibody.
Working concentration : 1 μg/ml
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REFERENCE
There are currently no references for NOX2 / gp91phox antibody (GTX31930). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NOX2 / gp91phox antibody (GTX31930). Be the first to share your experience with this product.
