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NPR-C antibody [2G2]

Overexpression
Anti-NPR-C antibody [2G2] used in Western Blot (WB). GTX84008
Anti-NPR-C antibody [2G2] used in IHC (Paraffin sections) (IHC-P). GTX84008
Anti-NPR-C antibody [2G2] used in IHC (Paraffin sections) (IHC-P). GTX84008
Anti-NPR-C antibody [2G2] used in Flow cytometry (FACS). GTX84008
Anti-NPR-C antibody [2G2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84008
Anti-NPR-C antibody [2G2] used in IHC (Paraffin sections) (IHC-P). GTX84008

Cat. No. GTX84008

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2G2

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all NPR-C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
IHC-P 1:50
FACS Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human NPR3 (NP_000899) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

natriuretic peptide receptor 3 , ANP-C , ANPR-C , ANPRC , C5orf23 , GUCY2B , NPR-C , NPRC

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.

Database

Research Area

DATA IMAGES

Anti-NPR-C antibody [2G2] used in Western Blot (WB). GTX84008

GTX84008 WB Image

Overexpression

WB analysis of HEK293T cells transfected with NPR-C plasmid (Right) or empty vector (Left) for 48 hrs using GTX84008 NPR-C antibody [2G2].
Loading : 5 ug per lane

Anti-NPR-C antibody [2G2] used in IHC (Paraffin sections) (IHC-P). GTX84008

GTX84008 IHC-P Image

IHC-P analysis of kidney tissue using GTX84008 NPR-C antibody [2G2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NPR-C antibody [2G2] used in IHC (Paraffin sections) (IHC-P). GTX84008

GTX84008 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84008 NPR-C antibody [2G2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-NPR-C antibody [2G2] used in Flow cytometry (FACS). GTX84008

GTX84008 FACS Image

FACS analysis of HEK293T cells transfected with either NPR-C plasmid(Red) or empty vector control plasmid(Blue) using GTX84008 NPR-C antibody [2G2].

Anti-NPR-C antibody [2G2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84008

GTX84008 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with NPR-C plasmid using GTX84008 NPR-C antibody [2G2].

Anti-NPR-C antibody [2G2] used in IHC (Paraffin sections) (IHC-P). GTX84008

GTX84008 IHC-P Image

IHC-P analysis of liver tissue using GTX84008 NPR-C antibody [2G2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519