GeneTex

  • Country / Location Selection

United States (US)

NR2C2 antibody

Orthogonal
Anti-NR2C2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105119
Anti-NR2C2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105119
Anti-NR2C2 antibody used in Western Blot (WB). GTX105119

Cat. No. GTX105119

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NR2C2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

H1299 , Molt-4

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NR2C2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951022

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor subfamily 2 group C member 2 , TAK1 , TR4

Cellular Localization

Nucleus

Background

Members of the nuclear hormone receptor family, such as NR2C2, act as ligand-activated transcription factors. The proteins have an N-terminal transactivation domain, a central DNA-binding domain with 2 zinc fingers, and a ligand-binding domain at the C terminus. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes (Yoshikawa et al., 1996 [PubMed 8661150]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NR2C2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105119

GTX105119 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NR2C2(GTX105119) antibody at 1:500 dilution.

Anti-NR2C2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105119

GTX105119 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using NR2C2(GTX105119) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NR2C2 antibody used in Western Blot (WB). GTX105119

GTX105119 WB Image

Orthogonal

Jurkat whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NR2C2 antibody (GTX105119) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for NR2C2 antibody (GTX105119). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for NR2C2 antibody (GTX105119). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169