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NRIP antibody

Orthogonal
Anti-NRIP antibody used in Western Blot (WB). GTX105954
Anti-NRIP antibody used in IHC (Paraffin sections) (IHC-P). GTX105954
Anti-NRIP antibody used in IHC (Paraffin sections) (IHC-P). GTX105954

Cat. No. GTX105954

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NRIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

HeLaS3 , HepG2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NRIP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950596

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DDB1 and CUL4 associated factor 6 , 1200006M05Rik , ARCAP , IQWD1 , MSTP055 , NRIP , PC326

Cellular Localization

Nucleus

Background

Ligand-dependent coactivator of nuclear receptors. Enhance transcriptional activity of the nuclear receptors NR3C1 and AR. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.

Database

Research Area

DATA IMAGES

Anti-NRIP antibody used in Western Blot (WB). GTX105954

GTX105954 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
B:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX105954 diluted at 1:1000

Anti-NRIP antibody used in IHC (Paraffin sections) (IHC-P). GTX105954

GTX105954 IHC-P Image

Orthogonal

The data was published in the journal Oncotarget in 2017. PMID: 28212551

Anti-NRIP antibody used in IHC (Paraffin sections) (IHC-P). GTX105954

GTX105954 IHC-P Image

The data was published in the journal Oncotarget in 2017.PMID: 28212551

REFERENCE

Application Reference

Tsai LK et al. J Cachexia Sarcopenia Muscle 2021; 12 (3) : 665-676 Autoantibody of NRIP, a novel AChR-interacting protein, plays a detrimental role in myasthenia gravis.
Application : IHC-Fr
Reactivity : Mouse
Chen HH et al. Oncotarget 2017; 8 (13) : 21501-21515 NRIP/DCAF6 stabilizes the androgen receptor protein by displacing DDB2 from the CUL4A-DDB1 E3 ligase complex in prostate cancer.
Application : IHC-P
Reactivity : Human
Chang SW et al. J Virol 2011; 85 (13) : 6750-63 NRIP, a novel calmodulin binding protein, activates calcineurin to dephosphorylate human papillomavirus E2 protein.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169