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NSD3 antibody [2E9]

Anti-NSD3 antibody [2E9] used in Western Blot (WB). GTX50055

Cat. No. GTX50055

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2E9

Isotype

 IgG2b

Application

 WB, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all NSD3 products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000 ~ 3,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

162 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human NSD3 (383-660aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor binding SET domain protein 3 , KMT3F , KMT3G , WHISTLE , WHSC1L1 , pp14328

Cellular Localization

Nucleus

Background

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. [provided by RefSeq, May 2015]

Database

Research Area

DATA IMAGES

Anti-NSD3 antibody [2E9] used in Western Blot (WB). GTX50055

GTX50055 WB Image

Cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human NSD3 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399