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NUP214 antibody

Anti-NUP214 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23349

Cat No. GTX23349

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, ELISA
Reactivity Human, Rat
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
ICC/IF 1:1000
IP Assay dependent
ELISA 1:15,000
Not tested in other applications.

Calculated MW

214 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Serum diluted in PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Peptide corresponding to amino acid residues 1881-2173 of rat cain.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nucleoporin 214,Cain,Can,Nup214

Cellular Localization

Nuclear pore complex,Nucleus

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES
Anti-NUP214 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23349

GTX23349 ICC/IF Image

ICC/IF analysis of NRK cells using GTX23349 NUP214 antibody.
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes
Dilution : 20 µg/ml in staining buffer for 1 hour at room temperature

REFERENCE
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Package List Price ($)
$ 319