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Nanog ELISA Pair

Nanog ELISA Pair. GTX500017

Cat. No. GTX500017

Application

 ELISA, Sandwich ELISA

Reactivity

 Human
Package
1 pair ($399)
See all Nanog ELISA Pair products
PRODUCT

Product Content

GTX100863
Type : Capture Antibody
Host : Rabbit
Conjugation : Unconjugated
Package : 50 μl
GTX89079
Type : Detection Antibody
Host : Goat
Conjugation : Unconjugated
Package : 50 μl

APPLICATION

Application Note

We recommend using the capture antibody at a concentration of 5μg/mL and the detection antibody at a concentration of 5μg/mL. Optimal dilutions should be determined experimentally by the researcher.

PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Nanog Homeobox , Nanog

Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Research Area

DATA IMAGES

Nanog ELISA Pair. GTX500017

GTX500017 ELISA Image

ELISA detection of NANOG using GTX100863 for capture at a concentration of 5 μg/mL and GTX89079 for detection at a concentration of 5 μg/mL. The HRP-conjugated anti-goat IgG antibody (GTX26741) was diluted at 1:20,000-1:200,000 and used to detect the primary antibody.

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Package List Price ($)
$ 399