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Nanog antibody [GT3312]

GTX627421_40878_WB_siRNA_18121411_763.jpg
GTX627421_40878_WB_M_ESC_18121411_534.jpg
GTX627421_40878_CT_FACS_18121411_114.jpg
GTX627421_40878_WB_18121411_915.jpg
GTX627421_40878_WB_hESC_18121411_657.jpg

Cat No. GTX627421

Host Mouse
Clonality Monoclonal
Clone Name GT3312
Isotype IgG2b
Application WB, ICC/IF, FACS
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , human ESC , mouse ESC , Tera-2

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nanog Homeobox,Nanog

Cellular Localization

Nucleus

Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Database

Research Area

DATA IMAGES
GTX627421_40878_WB_siRNA_18121411_763.jpg

GTX627421 WB Image

NANOG antibody [GT3312] validation by siRNA knock-down.
Upperpanel: NANOG antibody [GT3312] GTX627421
Lower panel: GAPDH antibody (GTX100118)
A. 30 µg Tera-2 whole cell lysate/extract
B. 30 µg whole cell lysate/extract of NANOG siRNA#1-transfected Tera-2 cells
C. 30 µg whole cell lysate/extract of NANOG siRNA#2-transfected Tera-2 cells
D. 30 µg whole cell lysate/extract of NANOG siRNA#3-transfected Tera-2 cells
10% SDS-PAGE
NANOG antibody [GT3312] (GTX627421) dilution: 1:1000
GAPDH antibody (GTX100118) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627421_40878_WB_M_ESC_18121411_534.jpg

GTX627421 WB Image

Sample (20 µg of whole cell lysate)
A: mouse ESC
10% SDS PAGE
GTX627421 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627421_40878_CT_FACS_18121411_114.jpg

GTX627421 FACS Image

NANOG antibody [GT3312] detects NANOG protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: NANOG antibody [GT3312] dilution: 1:50

GTX627421_40878_WB_18121411_915.jpg

GTX627421 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX627421 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627421_40878_WB_hESC_18121411_657.jpg

GTX627421 WB Image

NANOG antibody [GT3312] detects NANOG protein by western blot analysis.
A. 30 µg human ESC whole cell lysate/extract
10% SDS-PAGE
NANOG antibody [GT3312] (GTX627421) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Nanog antibody [GT3312] Cat No. GTX627421

Rating ( Average 4 based on 1 users reviews)
Application
Flow cytometry(FACS) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 11-Mar-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : hES cells
Blocking : 2% serum, 4°C, 0.5Hr
Fixation : 70% ethanol
Primary Antibody : 1:50, 4°C, 0.5Hr
Figure Note : Black: Isotype control, Green: NANOG antibody
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169