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NeuN antibody

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX37604
Anti-NeuN antibody used in Western Blot (WB). GTX37604
Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX37604

Cat. No. GTX37604

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all NeuN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-400
IHC-P 1:100-500 (based on 0.5 mg/ml)
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide derived from human NeuN. Please contact us for the exact immunogen sequence.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_11174267

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA binding fox-1 homolog 3 , FOX-3 , FOX3 , HRNBP3 , NEUN

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]

Database

Research Area

DATA IMAGES

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX37604

GTX37604 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX37604 NeuN antibody.
Dilution : 2.5μg/ml

Anti-NeuN antibody used in Western Blot (WB). GTX37604

GTX37604 WB Image

WB analysis of rat spinal cord (Lane 1), mouse brain (Lane 2), 293T cells (Lane 3) lysates using GTX37604 NeuN antibody.
Dilution : 2.5μg/ml

Anti-NeuN antibody used in IHC (Paraffin sections) (IHC-P). GTX37604

GTX37604 IHC-P Image

IHC-P analysis of rat spinal cord tissue using GTX37604 NeuN antibody.
Dilution : 2.5μg/ml

REFERENCE

Application Reference

Chao PK et al. bioRxiv 2017; (Epub) Drug discovery to counteract antinociceptive tolerance with mu-opioid receptor endocytosis.
Application : IHC-Fr
Reactivity : Mouse
Lee PT et al. Nucleic Acids Res 2014; Morphine drives internal ribosome entry site-mediated hnRNP K translation in neurons through opioid receptor-dependent signaling.
Application : WB
Reactivity : Human
Thomas MG et al. Cell Death Dis 2013; 4 : e669 Nicotinamide N-methyltransferase expression in SH-SY5Y neuroblastoma and N27 mesencephalic neurones induces changes in cell morphology via ephrin-B2 and Akt signalling.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399