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Neurofascin antibody

Anti-Neurofascin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133266
Anti-Neurofascin antibody used in IHC (Paraffin sections) (IHC-P). GTX133266
Anti-Neurofascin antibody used in Western Blot (WB). GTX133266
Anti-Neurofascin antibody used in IHC (Frozen sections) (IHC-Fr). GTX133266
Anti-Neurofascin antibody used in IHC (Paraffin sections) (IHC-P). GTX133266

Cat. No. GTX133266

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Neurofascin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

138 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of mouse Neurofascin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886899

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurofascin , AA387016 , D430023G06Rik , NF , mKIAA0756

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-Neurofascin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133266

GTX133266 ICC/IF Image

Neurofascin antibody detects Neurofascin protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Neurofascin protein stained by Neurofascin antibody (GTX133266) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Neurofascin antibody used in IHC (Paraffin sections) (IHC-P). GTX133266

GTX133266 IHC-P Image

Neurofascin antibody detects Neurofascin protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Neurofascin antibody (GTX133266) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Neurofascin antibody used in Western Blot (WB). GTX133266

GTX133266 WB Image

Various tissue extracts (10 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Neurofascin antibody (GTX133266) diluted at 1:2000.

Anti-Neurofascin antibody used in IHC (Frozen sections) (IHC-Fr). GTX133266

GTX133266 IHC-Fr Image

Neurofascin antibody detects Neurofascin protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Neurofascin protein stained by Neurofascin antibody (GTX133266) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Neurofascin antibody used in IHC (Paraffin sections) (IHC-P). GTX133266

GTX133266 IHC-P Image

Neurofascin antibody detects Neurofascin protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Neurofascin antibody (GTX133266) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169