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Neurogenin 1 antibody [10B10]

Overexpression
Anti-Neurogenin 1 antibody [10B10] used in Immunoprecipitation (IP). GTX84049
Anti-Neurogenin 1 antibody [10B10] used in Western Blot (WB). GTX84049
Anti-Neurogenin 1 antibody [10B10] used in Western Blot (WB). GTX84049

Cat. No. GTX84049

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10B10

Isotype

 IgG1

Application

 WB, IP

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all Neurogenin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IP 2-4ug/mg
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human NEUROG1 (NP_006152) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurogenin 1 , AKA , Math4C , NEUROD3 , bHLHa6 , ngn1

Cellular Localization

Nucleus

Background

Appears to mediate neuronal differentiation.

Database

Research Area

DATA IMAGES

Anti-Neurogenin 1 antibody [10B10] used in Immunoprecipitation (IP). GTX84049

GTX84049 IP Image

IP analysis of DDDDK tagged Neurogenin 1 overexpressed HEK293T lysate using GTX84049 Neurogenin 1 antibody [10B10].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-Neurogenin 1 antibody [10B10] used in Western Blot (WB). GTX84049

GTX84049 WB Image

WB analysis of various cell lines using GTX84049 Neurogenin 1 antibody [10B10].
Loading : 35 ug per lane

Anti-Neurogenin 1 antibody [10B10] used in Western Blot (WB). GTX84049

GTX84049 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Neurogenin 1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84049 Neurogenin 1 antibody [10B10].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519