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Neurotrophin 3 antibody [3D4]

Overexpression
Anti-Neurotrophin 3 antibody [3D4] used in Western Blot (WB). GTX83984
Anti-Neurotrophin 3 antibody [3D4] used in Western Blot (WB). GTX83984
Anti-Neurotrophin 3 antibody [3D4] used in IHC (Paraffin sections) (IHC-P). GTX83984
Anti-Neurotrophin 3 antibody [3D4] used in Western Blot (WB). GTX83984
Anti-Neurotrophin 3 antibody [3D4] used in IHC (Paraffin sections) (IHC-P). GTX83984

Cat. No. GTX83984

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3D4

Isotype

 IgG2a

Application

 WB, IHC-P

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all Neurotrophin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:10000
IHC-P 1:50
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 139-257 of human NT3 (NP_002518) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurotrophin 3 , HDNF , NGF-2 , NGF2 , NT-3 , NT3

Cellular Localization

Secreted

Background

Seems to promotes the survival of visceral and proprioceptive sensory neurons.

Database

Research Area

DATA IMAGES

Anti-Neurotrophin 3 antibody [3D4] used in Western Blot (WB). GTX83984

GTX83984 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Neurotrophin 3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83984 Neurotrophin 3 antibody [3D4].
Loading : 5 ug per lane

Anti-Neurotrophin 3 antibody [3D4] used in Western Blot (WB). GTX83984

GTX83984 WB Image

WB analysis of various cell lines using GTX83984 Neurotrophin 3 antibody [3D4].
Loading : 35 ug per lane

Anti-Neurotrophin 3 antibody [3D4] used in IHC (Paraffin sections) (IHC-P). GTX83984

GTX83984 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83984 Neurotrophin 3 antibody [3D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Neurotrophin 3 antibody [3D4] used in Western Blot (WB). GTX83984

GTX83984 WB Image

WB analysis of recombinant human NT3 using GTX83984 Neurotrophin 3 antibody [3D4].
Dilution : 1:10000

Anti-Neurotrophin 3 antibody [3D4] used in IHC (Paraffin sections) (IHC-P). GTX83984

GTX83984 IHC-P Image

IHC-P analysis of human liver tissue using GTX83984 Neurotrophin 3 antibody [3D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

Application Reference

NA Omar et al. Front Pharmacol 2023; 14 : 1307447 Neuroprotective effects of Neurotrophin-3 in MPTP-induced zebrafish Parkinson’s disease model
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519