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Nidogen 1 antibody [C1C3]

Anti-Nidogen 1 antibody [C1C3] used in Western Blot (WB). GTX102897

Cat. No. GTX102897

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Nidogen 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nidogen 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950998

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nidogen 1 , NID

Cellular Localization

Secreted , extracellular space , extracellular matrix , basement membrane

Background

This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Nidogen 1 antibody [C1C3] used in Western Blot (WB). GTX102897

GTX102897 WB Image

Nidogen antibody detects Nidogen protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5 % SDS-PAGE, and blotted with Nidogen antibody (GTX102897) diluted by 1:500

REFERENCE

Application Reference

Behrens DT et al. J Biol Chem 2012; 287 (22) : 18700-9 The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169