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Nitrotyrosine antibody [39B6]

Anti-Nitrotyrosine antibody [39B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX41979
Anti-Nitrotyrosine antibody [39B6] used in IHC (Paraffin sections) (IHC-P). GTX41979
Anti-Nitrotyrosine antibody [39B6] used in Western Blot (WB). GTX41979

Cat. No. GTX41979

Reference 8

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 39B6

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA

Reactivity

 Species independent
Package
100 μg ($409)
See all Nitrotyrosine products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1400
ICC/IF Assay dependent
IHC-P 1:100
IHC-Fr Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Product Note

Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Hybridoma line 39B6

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_10727902

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Nitrotyrosine antibody [39B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX41979

GTX41979 ICC/IF Image

IHC-P analysis of rat liver tissue using GTX41979 Nitrotyrosine antibody [39B6].

Anti-Nitrotyrosine antibody [39B6] used in IHC (Paraffin sections) (IHC-P). GTX41979

GTX41979 IHC-P Image

Nitrotyrosine tested on Bouin's fixed paraffin-embedded backskin sections of transgenic mice using a dilution of GTX41979

Anti-Nitrotyrosine antibody [39B6] used in Western Blot (WB). GTX41979

GTX41979 WB Image

Nitrotyrosine visualized on a retinal injury model using GTX41979.

REFERENCE

Application Reference

YN Huang et al. Cell Transplant 2024; 33 : 9636897241237049 Pomalidomide Improves Motor Behavioral Deficits and Protects Cerebral Cortex and Striatum Against Neurodegeneration Through a Reduction of Oxidative/Nitrosative Damages and Neuroinflammation After Traumatic Brain Injury
Poole LG et al. Blood 2021; 137 (18) : 2520-2531 Factor XIII cross-links fibrin(ogen) independent of fibrin polymerization in experimental acute liver injury.
Application : IP
Chen GP et al. J Diabetes Res 2020; 2020 : 7574245 Geranylgeranyl Transferase-I Knockout Inhibits Oxidative Injury of Vascular Smooth Muscle Cells and Attenuates Diabetes-Accelerated Atherosclerosis.
Application : IHC-Fr

Clone Reference

Liu D et al. Sci Adv 2020; 6 (41) ROS-responsive chitosan-SS31 prodrug for AKI therapy via rapid distribution in the kidney and long-term retention in the renal tubule.
M Nagaoka et al. Antioxidants (Basel). 2019; 8 (9):386 A Delphinidin-Enriched Maqui Berry Extract Improves Bone Metabolism and Protects against Bone Loss in Osteopenic Mouse Models
Application : ICC/IF
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REVIEW

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Application Reference
YN Huang et al. Cell Transplant 2024; 33:9636897241237049 Pomalidomide Improves Motor Behavioral Deficits and Protects Cerebral Cortex and Striatum Against Neurodegeneration Through a Reduction of Oxidative/Nitrosative Damages and Neuroinflammation After Traumatic Brain Injury
Poole LG et al. Blood 2021; 137 (18):2520-2531 Factor XIII cross-links fibrin(ogen) independent of fibrin polymerization in experimental acute liver injury.
Application : IP
Chen GP et al. J Diabetes Res 2020; 2020:7574245 Geranylgeranyl Transferase-I Knockout Inhibits Oxidative Injury of Vascular Smooth Muscle Cells and Attenuates Diabetes-Accelerated Atherosclerosis.
Application : IHC-Fr
Clone Reference
Liu D et al. Sci Adv 2020; 6 (41) ROS-responsive chitosan-SS31 prodrug for AKI therapy via rapid distribution in the kidney and long-term retention in the renal tubule.
M Nagaoka et al. Antioxidants (Basel). 2019; 8 (9):386 A Delphinidin-Enriched Maqui Berry Extract Improves Bone Metabolism and Protects against Bone Loss in Osteopenic Mouse Models
Application : ICC/IF
Boscolo Papo M et al. Fish Shellfish Immunol 2014; 39 (1):42-50 Histopathology and stress biomarkers in the clam Venerupis philippinarum from the Venice Lagoon (Italy).
Pascoli F et al. Aquat Toxicol 2011; 101 (43163):512-20 Evaluation of oxidative stress biomarkers in Zosterisessor ophiocephalus from the Venice Lagoon, Italy.
D Hof et al. Hum Antibodies 2011; 20 (1-2):15-27 Generation of novel recombinant antibodies against nitrotyrosine by antibody phage display
Application : ELISA
SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 409