We recommend using the capture antibody at a concentration of 5μg/mL and the detection antibody at a concentration of 1.5μg/mL. Optimal dilutions should be determined experimentally by the researcher.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development. [provided by RefSeq]
ELISA detection of NODAL using GTX106644 for capture at a concentration of 5 µg/mL and GTX88954 for detection at a concentration of 1.5 µg/mL. The HRP-conjugated anti-goat IgG antibody (GTX26741) was diluted at 1:20,000-1:200,000 and used to detect the primary antibody.
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