GeneTex
United States (US)

Noggin recombinant protein

Noggin-recombinant-protein-GTX65301-WB-1_18121411_965.jpg

Cat No. GTX65301

Application WB, ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

Protein's bioactivity determined by its ability to inhibit 5 ng/ml of BMP-4-induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for the effect is 0.05-0.08 ug/ml of Noggin

Calculated MW

23.1 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Storage

Reconstitute in water to a concentration of 0.1 to 1.0 mg/ml. Note: Due to solubility reasons the protein should be kept at low pH. This solution can then be diluted into other aqueous buffers. Store at -20ºC.

Antigen Species

Human

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥95%) and by HPLC (≥95%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

none

Background

Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-beta family and regulate their activity by inhibiting their access to signaling receptors. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. Recombinant human Noggin is a 23.1 kDa non-disulfide-linked homodimer consisting of a total of 206 amino acid residues.

Research Area

DATA IMAGES
Noggin-recombinant-protein-GTX65301-WB-1_18121411_965.jpg

GTX65301 WB Image

Purity of recombinant human Noggin (2 µg) was analyzed by reduced SDS-PAGE electrophoresis.

Package List Price ($)
$ 259