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Nop1p antibody [28F2]

Anti-Nop1p antibody [28F2] used in Western Blot (WB). GTX24575

Cat. No. GTX24575

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 28F2

Isotype

 IgG3

Application

 WB, ICC/IF, IP

Reactivity

 Yeast
Package
250 μl ($399)
See all NOP1 products

APPLICATION

Application Note

IF: Use at a dilution of 1/2000 - 1/20000 (yeast cells). IP: Use 2-4 μl for an amount of lysate derived from 5 OD600 units of yeast culture. WB: Use at a dilution of 1/10000 (cell lysates)- 1/25000 (nuclear fractions)(yeast protein samples). Predicted molecular weight: 34 kDa. For other (non-ECL) western detection methods, use at a dilution of 1/1000 - 1/5000. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

10mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Yeast

Immunogen

Nuclear preparation (S. cerevisiae).Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates.

Purification

Tissue culture supernatant
Sterile filtered.

Conjugation

Unconjugated

RRID

AB_370465

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Nucleus, nucleolus

Database

Research Area

DATA IMAGES

Anti-Nop1p antibody [28F2] used in Western Blot (WB). GTX24575

GTX24575 WB Image

Western blots of whole yeast protein extracts with a collection of our antibodies. The blot for GTX24575 is in the indicated lane, and the number indicates the SDS-PAGE molecular weight in kDa.

REFERENCE

Application Reference

Hayashi S et al. Nucleic Acids Res 2019; (Epub) Impact of intron removal from tRNA genes on Saccharomyces cerevisiae.
Application : ICC/IF
Reactivity : Yeast
Mochida K et al. Nature 2015; 522 (7556) : 359-62 Receptor-mediated selective autophagy degrades the endoplasmic reticulum and the nucleus.
Application : ICC/IF
Reactivity : Yeast
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399