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Noxa antibody

Orthogonal
Anti-Noxa antibody used in Western Blot (WB). GTX85521
Anti-Noxa antibody used in IHC (Paraffin sections) (IHC-P). GTX85521
Anti-Noxa antibody used in IHC (Paraffin sections) (IHC-P). GTX85521
Anti-Noxa antibody used in IHC (Paraffin sections) (IHC-P). GTX85521
Anti-Noxa antibody used in Western Blot (WB). GTX85521
Anti-Noxa antibody used in Western Blot (WB). GTX85521
Anti-Noxa antibody used in Western Blot (WB). GTX85521

Cat. No. GTX85521

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Noxa products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
IHC-P 1 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Noxa antibody was raised against a synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa.The immunogen is located within the first 50 amino acids of Noxa.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10725878

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phorbol-12-myristate-13-acetate-induced protein 1 , Noxa

Cellular Localization

Mitochondrion

Background

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified , which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9 . Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53 (1-3). Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development (4).

Database

Research Area

DATA IMAGES

Anti-Noxa antibody used in Western Blot (WB). GTX85521

GTX85521 WB Image

WB analysis of human stomach tissue lysate using GTX85521 Noxa antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

Anti-Noxa antibody used in IHC (Paraffin sections) (IHC-P). GTX85521

GTX85521 IHC-P Image

IHC-P analysis of human stomach tissue using GTX85521 Noxa antibody.
Working concentration : 1 μg/ml

Anti-Noxa antibody used in IHC (Paraffin sections) (IHC-P). GTX85521

GTX85521 IHC-P Image

IHC-P analysis of human stomach tissue using GTX85521 Noxa antibody.
Working concentration : 10 μg/ml

Anti-Noxa antibody used in IHC (Paraffin sections) (IHC-P). GTX85521

GTX85521 IHC-P Image

IHC-P analysis of human stomach tissue using GTX85521 Noxa antibody.
Working concentration : 2.5 μg/ml

Anti-Noxa antibody used in Western Blot (WB). GTX85521

GTX85521 WB Image

Orthogonal

The data was published in the journal PLoS One in 2017. PMID: 29045486

Anti-Noxa antibody used in Western Blot (WB). GTX85521

GTX85521 WB Image

Orthogonal

The data was published in the journal PLoS One in 2017. PMID: 29045486

Anti-Noxa antibody used in Western Blot (WB). GTX85521

GTX85521 WB Image

Orthogonal

The data was published in the journal PLoS One in 2017. PMID: 29045486

REFERENCE

Application Reference

Zera K et al. PLoS One 2017; 12 (10) : e0186707 Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399