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Nuclear Matrix Protein p84 antibody [5E10]

GTX70220_43264_20180928_IHC-P_18121411_272.jpg
p84-antibody-5E10-GTX70220-WB-1_18121411_975.jpg
GTX70220_43264_20180905_ICC_IF_18121411_771.jpg
GTX70220_41295_WB_18121411_757.jpg
GTX70220_41295_IP_18121411_947.jpg
p84-antibody-5E10-GTX70220-WB-7_18121411_221.jpg
p84-antibody-5E10-GTX70220-WB-5_18121411_879.jpg
p84-antibody-5E10-GTX70220-WB-3_18121411_751.jpg
p84-antibody-5E10-GTX70220-WB-6_18121411_506.jpg

Cat No. GTX70220

Host Mouse
Clonality Monoclonal
Clone Name 5E10
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IP, ChIP assay, IHC
Reactivity Human, Mouse, Rat, Hamster, Monkey
APPLICATION

Application Note

Recommended Starting Dilutions: For ICC/IF: Use at a concentration of 0.5 - 2 μg/ml. For IP: 1:100-1:500. For WB: Use at a concentration of 0.3 - 2 μg/ml. For ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

84 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , HeLa nucleus , NIH-3T3 , mouse brain , PC-12 , mouse Cerebellum

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:25296641)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Amino acids 15-374 of human p84 expressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tho Complex 1,Hpr1,P84,P84N5,Thoc1

Cellular Localization

Nuclear

Background

P84/N5-5E10 recognizes an 84 kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.

Database

Research Area

DATA IMAGES
GTX70220_43264_20180928_IHC-P_18121411_272.jpg

GTX70220 IHC-P Image

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p84-antibody-5E10-GTX70220-WB-1_18121411_975.jpg

GTX70220 WB Image

Detection of human p84/N5 in nuclear fraction by anti-p84/N5 5E10 monoclonal antibody (GTX70220) in western blot experiment. Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.

GTX70220_43264_20180905_ICC_IF_18121411_771.jpg

GTX70220 ICC/IF Image

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

GTX70220_41295_WB_18121411_757.jpg

GTX70220 WB Image

p84 antibody [5E10] detects p84 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
E. 30 µg A375 whole cell lysate/extract
7.5% SDS-PAGE
p84 antibody [5E10] (GTX70220) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70220_41295_IP_18121411_947.jpg

GTX70220 IP Image

p84 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 μg of p84 antibody [5E10] (GTX70220) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (GTX70220) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221667-01) was used as a secondary reagent.

p84-antibody-5E10-GTX70220-WB-7_18121411_221.jpg

GTX70220 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
B: NIH-3T3
C: mouse brain
D: PC-12
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

p84-antibody-5E10-GTX70220-WB-5_18121411_879.jpg

GTX70220 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
B: HeLa nucleus
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

p84-antibody-5E10-GTX70220-WB-3_18121411_751.jpg

GTX70220 WB Image

Sample (50 µg of whole cell lysate)
A: mouse Cerebellum
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

p84-antibody-5E10-GTX70220-WB-6_18121411_506.jpg

GTX70220 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Nuclear Matrix Protein p84 antibody [5E10] Cat No. GTX70220

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 06-Feb-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 30μg
Blocking : TBST with 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319