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Nucleostemin antibody [2C8D5]

Anti-Nucleostemin antibody [2C8D5] used in Western Blot (WB). GTX60706
Anti-Nucleostemin antibody [2C8D5] used in Flow cytometry (FACS). GTX60706
Anti-Nucleostemin antibody [2C8D5] used in ELISA (ELISA). GTX60706
Anti-Nucleostemin antibody [2C8D5] used in Western Blot (WB). GTX60706

Cat. No. GTX60706

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2C8D5

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($409)
See all Nucleostemin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human Nucleostemin (AA: 1-226) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein nucleolar 3 , C77032 , E2IG3 , NNP47 , NS

Cellular Localization

Nucleus,Nucleolus

Background

The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-Nucleostemin antibody [2C8D5] used in Western Blot (WB). GTX60706

GTX60706 WB Image

WB analysis of HEK293 (1) and Nucleostemin (AA: 1-226)-hIgGFc transfected HEK293 (2) cell lysate using GTX60706 Nucleostemin antibody [2C8D5].

Anti-Nucleostemin antibody [2C8D5] used in Flow cytometry (FACS). GTX60706

GTX60706 FACS Image

FACS analysis of Jurkat cells using GTX60706 Nucleostemin antibody [2C8D5].
Green : Nucleostemin
Purple : negative control

Anti-Nucleostemin antibody [2C8D5] used in ELISA (ELISA). GTX60706

GTX60706 ELISA Image

ELISA analysis of antigen using GTX60706 Nucleostemin antibody [2C8D5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Nucleostemin antibody [2C8D5] used in Western Blot (WB). GTX60706

GTX60706 WB Image

WB analysis of NIH3T3 (1) and PC-3 (2) cell lysate using GTX60706 Nucleostemin antibody [2C8D5].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409