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P2X7 antibody

Anti-P2X7 antibody used in IHC (Paraffin sections) (IHC-P). GTX104288
Anti-P2X7 antibody used in IHC (Frozen sections) (IHC-Fr). GTX104288
Anti-P2X7 antibody used in IHC (Paraffin sections) (IHC-P). GTX104288

Cat. No. GTX104288

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all P2X7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Guinea pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human P2X7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951086

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

purinergic receptor P2X 7 , P2X7

Cellular Localization

Cell membrane

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-P2X7 antibody used in IHC (Paraffin sections) (IHC-P). GTX104288

GTX104288 IHC-P Image

P2X7 antibody detects P2X7 protein at cell membrane and cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
P2X7 antibody (GTX104288) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-P2X7 antibody used in IHC (Frozen sections) (IHC-Fr). GTX104288

GTX104288 IHC-Fr Image

P2X7 antibody detects P2X7 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: P2X7 protein stained by P2X7 antibody (GTX104288) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-P2X7 antibody used in IHC (Paraffin sections) (IHC-P). GTX104288

GTX104288 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using P2X7(GTX104288) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Hu QQ et al. Purinergic Signal 2023; 19 (1) : 29-41 Dorsal root ganglia P2X4 and P2X7 receptors contribute to diabetes-induced hyperalgesia and the downregulation of electroacupuncture on P2X4 and P2X7.
Application : IHC-Fr
Reactivity : Rat
Yan Wang et al. Mol Med Rep 2021; 24 (1) Role of P2X7R in eosinophilic and non?eosinophilic chronic rhinosinusitis with nasal polyps.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169