APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain
Predict Reactivity
Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.12 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human P2Y2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11178636
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
purinergic receptor P2Y2 , HP2U , P2RU1 , P2U , P2U1 , P2UR , P2Y2 , P2Y2R
Cellular Localization
Cell membrane; Multi-pass membrane protein
Background
The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is responsive to both adenosine and uridine nucleotides. It may participate in control of the cell cycle of endometrial carcinoma cells. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-P2Y2 antibody [N3C3] used in Western Blot (WB). GTX114609](/templates/default/js/lightbox/images/loading.gif "Anti-P2Y2 antibody [N3C3] used in Western Blot (WB). GTX114609")
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GTX114609 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (GTX114609) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114609 WB Image
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (GTX114609) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114609 ICC/IF Image
P2Y2 antibody [N3C3] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [N3C3] (GTX114609) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX114609 IP Image
Immunoprecipitation of P2Y2 protein from 293T whole cell extracts using 5 μg of P2Y2 antibody [N3C3] (GTX114609).
Western blot analysis was performed using P2Y2 antibody [N3C3] (GTX114609).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX114609 ICC/IF Image
P2Y2 antibody [N3C3] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [N3C3] (GTX114609) diluted at 1:250.Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for P2Y2 antibody [N3C3] (GTX114609). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for P2Y2 antibody [N3C3] (GTX114609). Be the first to share your experience with this product.
![Anti-P2Y2 antibody [N3C3] used in Western Blot (WB). GTX114609](/upload/website/prouct_img/small/GTX114609/GTX114609_45028_20230908_WB_M_brain_23091319_660.webp "Anti-P2Y2 antibody [N3C3] used in Western Blot (WB). GTX114609")
![Anti-P2Y2 antibody [N3C3] used in Western Blot (WB). GTX114609](/upload/website/prouct_img/small/GTX114609/GTX114609_45028_20230901_WB_B_23091319_301.webp "Anti-P2Y2 antibody [N3C3] used in Western Blot (WB). GTX114609")
![Anti-P2Y2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114609](/upload/website/prouct_img/small/GTX114609/GTX114609_44321_20220415_ICC_IF_w_23060518_720.webp "Anti-P2Y2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114609")
![Anti-P2Y2 antibody [N3C3] used in Immunoprecipitation (IP). GTX114609](/upload/website/prouct_img/small/GTX114609/GTX114609_40548_20150407_IP_w_23060518_693.webp "Anti-P2Y2 antibody [N3C3] used in Immunoprecipitation (IP). GTX114609")
![Anti-P2Y2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114609](/upload/website/prouct_img/small/GTX114609/GTX114609_45028_20231222_ICC_IF_R_24011618_533.webp "Anti-P2Y2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114609")
![Anti-P2Y2 antibody [N3C3] used in Western Blot (WB). GTX114609](/upload/website/prouct_img/normal/GTX114609/GTX114609_45028_20230908_WB_M_brain_23091319_660.webp "Anti-P2Y2 antibody [N3C3] used in Western Blot (WB). GTX114609")
![Anti-P2Y2 antibody [N3C3] used in Western Blot (WB). GTX114609](/upload/website/prouct_img/normal/GTX114609/GTX114609_45028_20230901_WB_B_23091319_301.webp "Anti-P2Y2 antibody [N3C3] used in Western Blot (WB). GTX114609")
![Anti-P2Y2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114609](/upload/website/prouct_img/normal/GTX114609/GTX114609_44321_20220415_ICC_IF_w_23060518_720.webp "Anti-P2Y2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114609")
![Anti-P2Y2 antibody [N3C3] used in Immunoprecipitation (IP). GTX114609](/upload/website/prouct_img/normal/GTX114609/GTX114609_40548_20150407_IP_w_23060518_693.webp "Anti-P2Y2 antibody [N3C3] used in Immunoprecipitation (IP). GTX114609")
![Anti-P2Y2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114609](/upload/website/prouct_img/normal/GTX114609/GTX114609_45028_20231222_ICC_IF_R_24011618_533.webp "Anti-P2Y2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114609")