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PABPC3 antibody

Anti-PABPC3 antibody used in Western Blot (WB). GTX114611
Anti-PABPC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX114611

Cat. No. GTX114611

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PABPC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Jurkat , Raji , K562

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PABPC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177168

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(A) binding protein cytoplasmic 3 , PABP3 , PABPL3 , tPABP

Cellular Localization

Cytoplasm

Background

Messenger RNA stability and translation initiation are extensively under the control of poly(A)-binding proteins (PABP). See PABPC1 (MIM 604679) for background information.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PABPC3 antibody used in Western Blot (WB). GTX114611

GTX114611 WB Image

Sample (30 ug of whole cell lysate)

A: Jurkat

B: Raji

C: K562

7.5% SDS PAGE

GTX114611 diluted at 1:1000

Anti-PABPC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX114611

GTX114611 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using PABPC3(GTX114611) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : ICC/IF, IP
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169