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PAF1 antibody

Anti-PAF1 antibody used in Western Blot (WB). GTX116580
Anti-PAF1 antibody used in IHC (Whole mount) (IHC-Wm). GTX116580

Cat. No. GTX116580

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all PAF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Human, Rat, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618995

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PAF1 homolog, Paf1/RNA polymerase II complex component , F23149_1 , PD2

Cellular Localization

Nucleus

Background

PAF1, parafibromin (CDC73; MIM 607393), LEO1 (MIM 610507), and CTR9 (MIM 609366) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]). The PAF complex also has a role in histone monoubiquitination (Zhu et al., 2005 [PubMed 16307923]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PAF1 antibody used in Western Blot (WB). GTX116580

GTX116580 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX116580 diluted at 1:1000

Anti-PAF1 antibody used in IHC (Whole mount) (IHC-Wm). GTX116580

GTX116580 IHC-Wm Image

PAF1 antibody detects Paf1 protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
PAF1 antibody (GTX116580) dilution: 1:200.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169