GeneTex
United States (US)

PASK antibody [mAB6]

GTX15824_324_ICC-IF_19011519_335.jpg
GTX15824_325_ICC-IF_19011519_617.jpg
GTX15824_326_ICC-IF_19011519_686.jpg

Cat No. GTX15824

Host Mouse
Clonality Monoclonal
Clone Name mAB6
Isotype IgG2a
Application WB, ICC/IF, IHC
Reactivity Human, Rat, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:10-1:200
IHC Assay dependent
Not tested in other applications.

Calculated MW

143 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant, GST tagged, PAS fusion protein expressed in E. coli.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pas Domain Containing Serine/Threonine Kinase,Paskin,Stk37,Pask

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Database

DATA IMAGES
GTX15824_324_ICC-IF_19011519_335.jpg

GTX15824 ICC/IF Image

ICC/IF analysis of COS7 cells using GTX15824 PASK antibody [mAB6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 °C

GTX15824_325_ICC-IF_19011519_617.jpg

GTX15824 ICC/IF Image

ICC/IF analysis of Hela cells using GTX15824 PASK antibody [mAB6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 °C

GTX15824_326_ICC-IF_19011519_686.jpg

GTX15824 ICC/IF Image

ICC/IF analysis of L6 cells using GTX15824 PASK antibody [mAB6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 °C

Package List Price ($)
$ 319