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PCDH12 antibody

Anti-PCDH12 antibody used in IHC (Paraffin sections) (IHC-P). GTX85342
Anti-PCDH12 antibody used in Western Blot (WB). GTX85342

Cat. No. GTX85342

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all PCDH12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

129 kDa. ( Note )

Positive Control

K562 Cell Lysate(GTX27911)

Product Note

This antibody is predicted to not cross-react with PCDH18.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PCDH12 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH12.The immunogen is located within amino acids 30 - 80 of PCDH12.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protocadherin 12 , VE-cadherin-2 , VECAD2

Cellular Localization

Cell junction

Background

Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 12 (PCDH12) was initially identified through PCR screening of mouse heart microvascular endothelial cell RNA; further experiments revealed its mRNA to be strongly expressed in highly vascularized organs such as lung and kidney, in addition to glycogen-rich trophoblasts in the placenta. PCDH12- mice are viable and fertile, but show reduced placental and embryonic sizes when compared to wild-type mice. Further studies showed significant expression changes in 2,289 genes, including those involved in tissue morphogenesis, angiogenesis, cell-matrix adhesion and migration, immune response and chromatin remodeling. This antibody is predicted to not cross-react with PCDH18.

Database

Research Area

DATA IMAGES

Anti-PCDH12 antibody used in IHC (Paraffin sections) (IHC-P). GTX85342

GTX85342 IHC-P Image

IHC-P analysis of rat lung tissue using GTX85342 PCDH12 antibody.
Working concentration : 5 μg/ml

Anti-PCDH12 antibody used in Western Blot (WB). GTX85342

GTX85342 WB Image

WB analysis of K562 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX85342 PCDH12 antibody.
Working concentration : 1 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399