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PCNA antibody

Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539
Anti-PCNA antibody used in Western Blot (WB). GTX100539
Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539
Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539
Anti-PCNA antibody used in Western Blot (WB). GTX100539
Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539
Anti-PCNA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100539
Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539
Anti-PCNA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100539
Anti-PCNA antibody used in Immunoprecipitation (IP). GTX100539
Anti-PCNA antibody used in Western Blot (WB). GTX100539

Cat No. GTX100539

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP, FISH, IHC
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
FISH Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , PC-12 , *A549 , *SiHa

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377373)

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

proliferating cell nuclear antigen,ATLD2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539

GTX100539 IHC-P Image

PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PCNA antibody (GTX100539) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PCNA antibody used in Western Blot (WB). GTX100539

GTX100539 WB Image

PCNA antibody detects PCNA protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
12% SDS-PAGE
PCNA antibody (GTX100539) dilution: 1:2500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539

GTX100539 IHC-P Image

PCNA antibody detects PCNA protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
PCNA antibody (GTX100539) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539

GTX100539 IHC-P Image

PCNA antibody detects PCNA protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
PCNA antibody (GTX100539) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PCNA antibody used in Western Blot (WB). GTX100539

GTX100539 WB Image

PCNA antibody detects PCNA protein by western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract
12% SDS-PAGE
PCNA antibody (GTX100539) dilution: 1:2500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539

GTX100539 IHC-P Image

PCNA antibody detects PCNA protein at nucleus in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
PCNA antibody (GTX100539) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PCNA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100539

GTX100539 ICC/IF Image

PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PCNA protein stained by PCNA antibody (GTX100539) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX100539

GTX100539 IHC-P Image

PCNA antibody detects PCNA protein at nucleus in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon .
PCNA antibody (GTX100539) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PCNA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100539

GTX100539 ICC/IF Image

PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PCNA protein stained by PCNA antibody (GTX100539) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PCNA antibody used in Immunoprecipitation (IP). GTX100539

GTX100539 IP Image

Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 μg of PCBP2 antibody (GTX100539).
Western blot analysis was performed using PCBP2 antibody (GTX100539).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PCNA antibody used in Western Blot (WB). GTX100539

GTX100539 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (GTX100539) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

PCNA antibody Cat No. GTX100539

Rating ( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 05-Jul-2018
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : PANC-1 cells
Blocking : PBS with 2% FBS and 0.4% Triton X-100, 25°C, 0.17Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : PBS with 2% FBS and 0.4% Triton X-100
Primary Antibody : 1:100, 4°C, 16Hr
Date : Anonymous submitted on 23-May-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 2Hr
Date : Anonymous submitted on 30-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells,SiHa cells,HeLa cells
Amount used : 10μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf