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PCNA antibody

Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX15497

Cat. No. GTX15497

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
500 μl ($399)
See all PCNA products

APPLICATION

Application Note

IHC-P: Use at a dilution of 1/200 for 10 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues. WB: Use at an assay dependent dilution. Predicted molecular weight: 31 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

29 kDa. ( Note )

Positive Control

Tonsil, lymph node, or small intestine

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human PCNA protein

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proliferating cell nuclear antigen , ATLD2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PCNA antibody used in IHC (Paraffin sections) (IHC-P). GTX15497

GTX15497 IHC-P Image

Human Tonsil stained with anti-PCNA antibody (GTX15497)

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399