IHC-P: Use at an assay dependent dilution. Staining of formalin fixed tissues requires boiling tissue sections in 10mMcitrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Optimal dilutions/concentrations should be determined by the end user.
Human skin and Ovarian Carcinoma
PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) corresponding to C terminal of human PDGFR alpha.
Immunogen affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Platelet Derived Growth Factor Receptor Alpha,Cd140A,Pdgfr-2,Pdgfr2,Pdgfra
Cell Membrane and Cytoplasmic.
Platelet derived growth factor receptor (PDGFR) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF AA, BB or AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras, Raf and ERK 1 and 2 pathway.