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PEF1 antibody [N2C3]

Anti-PEF1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106588
Anti-PEF1 antibody [N2C3] used in Western Blot (WB). GTX106588
Anti-PEF1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX106588

Cat. No. GTX106588

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PEF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PEF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951170

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

penta-EF-hand domain containing 1 , ABP32 , PEF1A

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1; MIM 114170), sorcin (SRI; MIM 182520), grancalcin (GCA; MIM 607030), and ALG2 (PDCD6; MIM 601057) (Kitaura et al., 2001 [PubMed 11278427]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PEF1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106588

GTX106588 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Peflin(GTX106588) antibody at 1:200 dilution.

Anti-PEF1 antibody [N2C3] used in Western Blot (WB). GTX106588

GTX106588 WB Image

Sample(30 ug whole cell lysate)
A:293T
12% SDS PAGE
GTX106588 diluted at 1:1000

Anti-PEF1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX106588

GTX106588 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using PEF1(GTX106588) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169