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PERP antibody

Anti-PERP antibody used in Western Blot (WB). GTX85520
Anti-PERP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85520

Cat. No. GTX85520

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all PERP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

A-431 Cell Slide(GTX25525) , A431 Cell Lysate(GTX27909)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PERP antibody was raised against a synthetic peptide corresponding to 17 amino acids near the carboxy terminus of human PERP.The immunogen is located within the last 50 amino acids of PERP.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PERP, TP53 apoptosis effector , 1110017A08Rik , KCP1 , KRTCAP1 , PIGPC1 , THW

Cellular Localization

Cell membrane

Background

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), PID/MTA2 (6), MTBP (7) and PERP (8), were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein (8-10). p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis (8). The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family (8).

Database

Research Area

DATA IMAGES

Anti-PERP antibody used in Western Blot (WB). GTX85520

GTX85520 WB Image

WB analysis of A431 cell lysate using GTX85520 PERP antibody in (A) the absence and (B) the presence of blocking peptide.
Dilution : 1 μg/ml

Anti-PERP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85520

GTX85520 ICC/IF Image

ICC/IF analysis of A431 cells using GTX85520 PERP antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399