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PHLDA3 antibody [4B6]

KO/KD Orthogonal
Anti-PHLDA3 antibody [4B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00700
Anti-PHLDA3 antibody [4B6] used in Western Blot (WB). GTX00700

Cat. No. GTX00700

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4B6

Isotype

 IgG2b

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μg ($399)
See all PHLDA3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to N terminal amino acids 1-31 of human PHLDA

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pleckstrin homology like domain family A member 3 , TIH1

Cellular Localization

Cytoplasm,Membrane

Database

Research Area

DATA IMAGES

Anti-PHLDA3 antibody [4B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00700

GTX00700 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00700 PHLDA3 antibody [4B6].
Dilution : 1:1000
Fixation : 4% PFA
Permeabilization: 0.25% Triton X-100 in PBS for 10 min

Anti-PHLDA3 antibody [4B6] used in Western Blot (WB). GTX00700

GTX00700 WB Image

KO/KD Orthogonal

WB analysis of MDA-MB-468 cell lysate using GTX00700 PHLDA3 antibody [4B6]. PHLDA3 protein was induced by Ad-p53 in MDA-MB-468 cells (left). PHLDA3 expression was inhibited by shRNA targeting PHLDA3 (right).

REFERENCE

Clone Reference

Kawase T et al. Cell. 2009; 136 (3):535-50 PH domain-only protein PHLDA3 is a p53-regulated repressor of Akt.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399